Granada Crystallization Box ^®

FOR PROTEIN CRYSTALLIZATION

Granada Crystallization Box^® (GCB) is a simple device to crystallize protein and other biological macromolecules by counter-diffusion method. GCB consists of 4 elements made of polystyrene:

• A reservoir to introduce the gel
• A guide to hold capillaries
• A cover
• A holder to maintain the boxes

The GCB has been designed to be used in four different ways: 

• To grow crystals inside gels under diffusion controlled mass transport.
• To grow protein crystals inside capillaries with un-gelled precipitating agent by the counter-diffusion technique.
• To grow protein crystals inside capillaries with gelled precipitating agent by the counter-diffusion technique.
• To grow protein crystals inside capillaries by the batch method.

Main characteristics:

• Crystallization is performed under diffusion-controlled mass transport.

The role of viscous fluids, gels and/or micro-gravity is enhanced by using capillary volumes.

• Reduces the consumption of macromolecule:

From 500 nanoliters to 100 nanoliters of protein solutions depending on the inner diameter of the capillaries.

• Automatically searches for the optimal crystallization conditions: 

One capillary scans the same precipitation region than hundreds of drops.

• Easy and safe transport to x-ray diffraction facilities 

Transport your precious crystals in your pocket!

• Full visualization of the crystallization process

• In situ x-ray crystallography: 

Crystals grow inside x-ray capillaries

If desired, no handling of crystals

Usable for cryo-crystallography

Triana Science & Technology offers GCBs in packages of 20 boxes

User guide of Granada Crystallization Box^® (GCB):   

To make an order, just send us an email to This e-mail address is being protected from spambots. You need JavaScript enabled to view it with a list of the required products and services you need or from the menu "orders". 

References

1. J.M. García-Ruiz, Counterdiffusion Methods for Macromolecular Crystallization. Methods in Enzymology, Vol. 368 (2003) 130-154. 
2. Granada Crystallisation Box: a new device for protein crystallisation by counter-diffusion techniques. Garcia-Ruiz JM, Gonzalez-Ramirez LA, Gavira JA, Otalora F. Acta Crystallogr D Biol Crystallogr. 2002 Oct;58 (Pt 10 Pt 1):1638-42.