Description

The Capillary Counterdiffusion Kits^® are the simplest and cleaver way to search for crystallization conditions.

Only 300 nanoliters of protein solution per experiment
No pipeting, no drops, no grease.
Each experiment is set in less tan 30 seconds (measured).
No handling of crystals: In situ X-ray characterization (proven with our glass capillaries).
Possible in situ full X-ray data collection for solving structure (demonstrated with our glass capillaries).

Each kit contains six GCB-Domino boxes filled with a precipitant solution or a mixture of precipitant buffered at six pH values. Just punch the 0.1 mm diameter capillaries filled with your protein solution. Counterdiffusion technique will do the work: an exhaustive testing of all precipitant concentrations at six different pH values and at different rates of development of supersaturation. In other words, Capillary Counterdiffusion will tell you everything about the crystallization behavior of your protein for a given precipitant or mixture of precipitants.

The same advantages of the Granada Crystallization Box (GCB) but now:

The GCB-Domino boxes are filled with precipitant solutions. You do not need to prepare either the solutions or the gel.
The volume of protein solution per experiment is now reduced down to 300 nanoliters.
Our quality tested capillaries can be used for in situ X-ray diffraction data collection.
The GCB-Domino boxes allow full visualization of the capillaries under microscope even when all the six boxes are located in their cardboard frame.

What you need to use the Capillary Counterdiffusion Kits:

Available Kits:

Custom-made Kits:

We also offer the posibility to order your own Capillary Counterdiffusion Kit with the conditions you need. Just send us an email to triana@trianatech.com with the conditions you need and we will prepare a Kit with such conditions.

To make an order, just send us an email to triana@trianatech.com with a list of the required products and services you need.

User Guide Capillary Counterdiffusion Kits

1. J.M. García-Ruiz, Counterdiffusion Methods for Macromolecular Crystallization. Methods in Enzymology, Vol. 368 (2003) 130-154.

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